Expression of Aspergillus oryzae Tannase in Pichia pastoris and Its Application in the Synthesis of Propyl Gallate in Organic Solvent
نویسندگان
چکیده
Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of Aspergillus oryzae tannase was achieved in Pichia pastoris under the control of the AOX1 promoter, and the productivity of recombinant tannase was 960 U/L or 64 U/g dry mass. The recombinant P. pastoris was used to synthesize propyl gallate in organic solvent and the yield of propyl gallate was 53 %.
منابع مشابه
Synthesis of antioxidant propyl gallate using tannase from Aspergillus niger van Teighem in nonaqueous media.
Tannase from Aspergillus niger van Teighem has been used for synthesis of food additive antioxidant propyl gallate by direct transesterification of tannic acid. The optimized yield of 86% was obtained by using simultaneously pH tuned enzyme, immobilized on Celite and using the right amount of water in the non aqueous media.
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